Leontina Grigore-Gurgu and Florentina Ionela Bucur returned from the Institute of Microbiology and  Biotehnology from the University of Ulm, Germany, where they had a hands-on training regarding genome editing using “gene knockout” based on CRISPRi and “gene deletion” based on chromosomal mutagenesis using two vectors (pORI280SacBmut and pVE6007), the second one being thermo-sensitive.